#SNAPGENE EF1A PLUS#
#SNAPGENE EF1A FREE#

TagRFP and TagGFP2 fluorescent markers PuroR, BleoR, NeoR, BlastR, and HygroR antibiotic-resistance markers mouse Thy1.1 & Thy1.Constitutive or inducible versions of U6 or H1 shRNA/sgRNA expression pol III promoters of 11 Sanger Sequencing Troubleshooting Guide (GNGFM00346) v1.Choose from a variety of vector elements, and we’ll build your vector: Ready-to-use Lentiviral Packaging Plasmid MixĬellecta can also customize vectors.CloneTracker XP™ Expressed Barcode pScribe Vectors.Thus, we only have individual clones of pLX317 with both a barcode and an ORF insert.

#SNAPGENE EF1A PLUS#

This is because pLX317, by definition, is pLX307 plus a barcode sequence, but this barcode is added in the same workflow as the ORF insert.

InDOXible™ Dox-Activated cDNA Expression System pLX317 is not available for distribution as a Gateway Destination vector, and users should request pLX307 instead.

Sticky ends from different RsrII sites may not be compatible. Efficient cleavage requires at least two copies of the RsrII recognition sequence. Sticky ends from different AlwNI sites may not be compatible.

CloneTracker XP™ Expressed sgRNA-Barcode and sgRNA-Only pRSGScribe Vectors Adeno-associated virus (AAV) vector for expressing a C-terminally TurboGFP-tagged protein using the EF-1a promoter.

dCas9-only expression for CRISPRa or CRISPRi, for use with Two-Vector System sgRNA-only vectors.

Delivery Volume 100 L Titer 1×10 vg/mL Pricing 350 USD for preparation of 100 L virus + 30 USD for plasmid. These AAV preparations are suitable purity for injection into animals.

Cas9-only expression for CRISPR knockout, for use with Two-Vector System sgRNA-only vectors EF1a-driven expression of cytosolic sRGECO calcium sensor.

Karl Deisseroths lab contains the insert hChR2(E123T/H134R)-EYFP and is published in Nat Neurosci.

Dual-sgRNA expression for CRISPR knockout, CRISPRa, or CRISPRi Plasmid pAAV-Ef1a-DIO ChETA-EYFP from Dr.

sgRNA-only expression for CRISPR knockout, CRISPRa, or CRISPRi (Two-Vector System).

sgRNA+Cas9 expression for Single-Vector System CRISPR knockout.

shRNA KnockdownĬRISPR Knockout, CRISPRa Activation, and CRISPRi Repression gb sequences as files rather than having them open up in the browser, right-click on the link and 'Save Link As.' or 'Download Linked File As.'. Linearized shRNA/sgRNA vectors or expression vectors with multiple cloning sites are also available, so you may clone your own inserts. Please contact us for information on a specific vector not listed or if interested in a customized vector.Ĭellecta’s vector collection includes the following types of expression vectors and accessory vectors. Please note that this list is not comprehensive.

#SNAPGENE EF1A FREE#

For a free vector file viewer, try ApE or SnapGene Viewer. Find all vector information here, including cloning/Next-Gen Sequencing (NGS) cassette and primer design maps, sequences, and vector maps in SnapGene (.dna), GenBank/Vector NTI (.gb), and FASTA (.txt) formats. pLXTRC307 Pol II Cassette 1: SV40-PuroR Pol II Cassette 2: EF1a-Gateway-V5(c-term). Here, we develop a comprehensive intersectional targeting toolbox including 39 novel vectors for joint-feature-targeted delivery of 13 molecular payloads (including opsins, indicators, and fluorophores), systematic approaches for development and optimization of new intersectional tools, hardware for in vivo monitoring of expression dynamics, and the first versatile single-virus tools (Triplesect) that enable targeting of triply defined cell types.Cellecta offers a comprehensive collection of cloning and expression vectors for individual constructs to pooled library generation. At the same time, genetically targeted technology for writing in and reading out activity patterns for cells in living organisms has enabled causal investigation in physiology and behavior however, cell-type-specific delivery of these tools (including microbial opsins for optogenetics and genetically encoded Ca 2+ indicators) has thus far fallen short of versatile targeting to cells jointly defined by many individually selected features.

The resolution and dimensionality with which biologists can characterize cell types have expanded dramatically in recent years, and intersectional consideration of such features (e.g., multiple gene expression and anatomical parameters) is increasingly understood to be essential.

Snapgene ef1a